Phytochemical screening and properties of botanical crude extracts and ethyl acetate fractions isolated by deep eutectic solvent

Botanicals, being safe because of their natural origin, are associated with potential health benefits due to isoflavones-rich content. Isoflavones as plant-generated secondary metabolites formed defend against environmental stress such UV radiation, mechanical damage, or any other attack from the surroundings. Kudzu (Pueraria roots and flowers), red clover (Trifolium pratense), soy (Glycine max) significant importance in medical field. were reported exhibit a variety biological functions, e.g., antiaging, anticancer, microbiome modulations. This study focuses attention on extraction quantification isoflavones kudzu (KR), flowers (KF), (RC), molasses (SM), determining total polyphenols (TPC), flavonoids (TFC), antioxidant activity (AOA). Novel green ultrasound-assisted (UAE) technology was developed extract quantify isoflavones. The results showed quantity KR, KF, RC, SM crude extracts be 1.01%, 2.9%, 0.70% 0.67%, respectively. fractionation using ethyl acetate (EA) improved yield that became 1.48%, 2.5%, 1.3% 0.89% RC fractions, investigation eventually our UAE methodology is efficient method for recovering isoflavone contents recorded follows: KFKRRCSM. We concluded these botanicals crucial source strong properties.